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Amaxa human monocyte nucleofector kit
Human Monocyte Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+monocyte+nucleofector+kit/human+kit+monocyte+nucleofector/bio_rxiv__64898__2026__02__11__705073-212-20-24
Average 86 stars, based on 1 article reviews
human monocyte nucleofector kit - by Bioz Stars, 2026-09
86/100 stars

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Article Title: Viral microRNA regulation of Akt is necessary for reactivation of Human Cytomegalovirus from latency in CD34 + hematopoietic progenitor cells and humanized mice
Article Snippet: The human serum was removed for transfection, and then the monocytes (3 × 10 6 ) were resuspended in 100 μl of room temperature nucleofection solution (human monocyte Nucleofector kit; Amaxa Biosystems, Cologne, Germany) containing a negative control small interfering RNA (siRNA) (scrambled control), a mixture of UL36+UL112+UL148D miRNAs, or an Akt siRNA (Ambion, Thermo Fisher Scientific, Waltham, MA).

Article Title: Viral microRNA regulation of Akt is necessary for reactivation of Human Cytomegalovirus from latency in CD34+ hematopoietic progenitor cells and humanized mice.
Article Snippet: The human serum was removed for transfection, and then the monocytes (3 × 106) were resuspended in 100 μl of room temperature nucleofection solution (human monocyte Nucleofector kit; Amaxa Biosystems, Cologne, Germany) containing a negative control small interfering RNA (siRNA) (scrambled control), a mixture of UL36+UL112 +UL148D miRNAs, or an Akt siRNA (Ambion, Thermo Fisher Scientific, Waltham, MA).

Transfection:

Article Title: GATA2 Mediates Macrophage Proliferation During Atherosclerosis
Article Snippet: .. Wild type THP‐1 or GATA2‐overexpressed THP‐1 cells were transfected with 0.5 μg of DNA using an Amaxa Human Monocyte Nucleofector kit and a Lonza Nucleofector II, using the V‐001 protocol. ..

Article Title: GATA2 Mediates Macrophage Proliferation During Atherosclerosis
Article Snippet: .. Wild type THP- 1 or GATA2- overexpressed THP- 1 cells were transfected with 0.5 μg of DNA using an Amaxa Human Monocyte Nucleofector kit and a Lonza Nucleofector II, using the V- 001 protocol. ..

Article Title: TACE reprograms RANKL-mediated differentiation of macrophages by activating the non-canonical pathway of IRF3
Article Snippet: RNA interference was performed using 0.2 nmol of three short interfering RNAs (siRNAs) targeting human TACE (Invitrogen; HSS186181) or a control siRNA (D-001810-10). .. These siRNAs were transfected into primary human CD14+ monocytes using the Amaxa Nucleofector device, set to program Y-001, with the Human Monocyte Nucleofector Kit (Amaxa), as previously described . .. For adenoviral transduction, recombinant adenoviral particles encoding human TACE (ADV-200349) or IRF3 (ADV-212405) and control (1060) adenoviral particles encoding green fluorescent protein (Ad-CMV-GFP) were purchased from Vector Biolabs (Malvern, PA, USA).

Article Title: THOC5 regulates human osteoclastogenesis
Article Snippet: .. RNA interference 0.2 nmol of small interfering RNAs (siRNAs), specifically targeting human THOC5 (Dharmacon, J-015317–09–0002 and J-015317–12–0002) or control siRNA (Dharmacon, D-001810–10) were transfected into primary human CD14 + monocytes with the Amaxa Nucleofector device, set to program Y-001 using the Human Monocyte Nucleofector kit, (Amaxa), as previously described ( ). .. 293 T cells were transfected with pCMV6-Entry-c-FMS-MYC-DDK (NM_005211, Origene, Rockville, MD) using Lipofectamine 3000 (ThermoFisher scientific) as previously described ( ).

Over Expression:

Article Title: ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Article Snippet: .. Stable overexpression cell line generation THP1 monocytic cell lines stably expressing ARL8B mutants were generated using Amaxa human monocyte nucleofector kit (VPA-1007). ..

Stable Transfection:

Article Title: ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Article Snippet: .. Stable overexpression cell line generation THP1 monocytic cell lines stably expressing ARL8B mutants were generated using Amaxa human monocyte nucleofector kit (VPA-1007). ..

Expressing:

Article Title: ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Article Snippet: .. Stable overexpression cell line generation THP1 monocytic cell lines stably expressing ARL8B mutants were generated using Amaxa human monocyte nucleofector kit (VPA-1007). ..

Generated:

Article Title: ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization.
Article Snippet: .. Stable overexpression cell line generation THP1 monocytic cell lines stably expressing ARL8B mutants were generated using Amaxa human monocyte nucleofector kit (VPA-1007). ..

Control:

Article Title: THOC5 regulates human osteoclastogenesis
Article Snippet: .. RNA interference 0.2 nmol of small interfering RNAs (siRNAs), specifically targeting human THOC5 (Dharmacon, J-015317–09–0002 and J-015317–12–0002) or control siRNA (Dharmacon, D-001810–10) were transfected into primary human CD14 + monocytes with the Amaxa Nucleofector device, set to program Y-001 using the Human Monocyte Nucleofector kit, (Amaxa), as previously described ( ). .. 293 T cells were transfected with pCMV6-Entry-c-FMS-MYC-DDK (NM_005211, Origene, Rockville, MD) using Lipofectamine 3000 (ThermoFisher scientific) as previously described ( ).



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